Effect of CO_2 Concentration on Net Photosynthetic Rate of Coffea xarabusta Somatic Embryos Cultured Photoautotrophically

摘要

Somatic embryos have been used for woody plant micropropagation. In Coffea, Afreen et al. (2001) showed that C. xarabusta cotyledonary embryos had photosyn-thetic ability and could be cultured photoautotrophically (sugar-free culture) (Kozai, 1991). Therefore, application of photoautotrophic culture from the cotyledonary embryo stage through the rest of the production stages potentially shortens the transplant production period and enhances the establishment of transplants ex vitro as compared with photomixo trophic culture (sugar-containing culture) In this study, effect of CO_2 concentration on the net photosynthetic rate of C. xarabusta somatic embryos was investigated to determine the environmental conditions for the photoautotrophic culture ofsomatic embryos.