Surveillance for influenza A virus (IAV) is central to the control of influenza in pigs and the prevention of zoonotic infections. However, routinely and readily detecting influenza infections can be challenging particularly in endemically infected herds where antibody levels are high, clinical signs are mild and virus levels fluctuate. Furthermore, control of IAV infections usually requires the successful recovery of virus isolates from collected samples with subsequent characterization, vaccine seedstock production, and diagnostic assay development from the isolates. In this study, we developed and compared novel sampling strategies to detect and isolate IAV in swine breeding herds.
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