Proteins modulate the properties of biomimetic calcium phosphate coatings on titanium implants

摘要

Calcium phosphate biomimetic coatings are made by mimicking the physiological process in which biomineralization takes place. We hypothesize that by adding small amounts of proteins into mineral of the coatings, could modulate its mechanical and dissolution properties, such as stiffness and strength, the Ca{sup}(2+) release and its mechanical adhesion to the metal substrate. Bovine serum albumin (PSA) was added over a wide range (0.01μg/ml - 1000.0 μg/ml) during the calcium phosphate coatings formation process and was deposited onto the surfaces of titanium alloy (Ti6Al4V) substrate. Titanium-alloy implants were coated with a layer of calcium phosphate that contains small amounts of protein. Micro scratch tests were performed in order to evaluate adhesion strength between the coating and the Ti6Al4V surfaces. The coating dissolution in saline solution was measured using a calcium ion specific electrode. Our results indicates that portein incorporated into biomimetic coatings modulate their properties such as coatings strength and dissolution rate.