Use of the sedimentation coefficient for modelling antibodies. Refinements to the crystallohydrodynamics approach

摘要

In a previous paper (Carrasco B, Garcia de la Torre J, Davis KG, Jones S, Athwal D, Walters C, Burton DR, Harding SE (2001) Biophysical Chemistry 93:181–196) we introduced the concept of Crystallohydrodynamics for modelling the conformation of antibodies in solution and allowing for the so-called hydration problem. We now make some further improvements to allow for the hydrated dimensions of the domains before assembling models for the intact antibody using the algorithms HYDROSUB and SOLPRO. The procedure is illustrated by application to a human immunoglobulin construct of the subclass IgG4 from the mouse IgG1 (4P).