Synchrotron-Tomography for Evaluation of Bone Tissue Regeneration using rapidly Resorbable Bone Substitute Materials

摘要

This article presents a novel approach to evaluate two-dimensional histomorphometric studies of biodegradable ceramic particles by means of element-sensitive, three-dimensional and non-destructive synchrotron-microtomography (SCT). An in vivo animal study was performed in which bone substitute materials (352i, GB9/25) were implanted in the sheep mandible to support the bone regeneration. After 12 and 24 weeks of implantation samples were prepared and investigated using SCT and subsequent 3D image analysis as well as histological evaluation. A comparison of corresponding tomographical and histological slices delivers information about the newly formed bone and its stage of development. Additionally SCT gives insights into the structural changes of the bony tissue in a given defect and the local biodegradation of the bone substitute material in a three-dimensional manner.