Fucoidanase production by marine fungus

摘要

In China, there are abound of Lominaria sp. produced annually. It is more important to develop these algae byproducts. Fucoidan, a kind of heteropolysaccharides, is mainly composed of sul fated L-fucose and a small proportion of galactose, mannose, xylose and uronic acids. It mainly exists on the surface of sea brown alga, mixing with alginate and laminaria. The fucoidan content of brown alga varies with the change of climate and species. In recent years, many investigated reports showed that fucoidan, especially low molecule weight fucan (LMWF in short ), had its clinic activities such as antivirus,, anticoagulant and antithrombic activity, and other activities. Fucoidan could be also used as drugs for antitumoral, antifertilizing and plasminogen. As fucoidan molecular weight is too large for use in drugs, low molecular weight fucans were prepared. There are some methods such as chemical acid hydrolysis, physical radical cleavage and biological enzyme hydrolysis for preparing low molecular weight fucans. Among them, biological method may be the best one. Fucoidanase can hydrolyze fucoidan to small fragment without removal of side substitute groups because of its substrate hydrolysis property. Few papers concerning fucoidanase production were reported. Fucoidanase could be obtained from hepatopancreas of animal and some bacteria such as Pseudomonas atlantica, Pseudomonas carrageenovora and Vibrio sp. N-5. A species marine fungus Dendryphiella arenaria TM94 were used to produce fucoidanase by solid state fermentation in our laboratory. In this paper, we described the production and properties of fucoidanase by Dendryphiella arenaria TM94.