ANTIGEN-ANTIBODY INTERACTION ON THE GOLD SURFACE MODIFIED BY LANGMUIR-SHAEFFER TECHNOLOGIES WITH POLY-PYRROLE-DERIVATIVES MONITORED BY μLIBRA

摘要

Here we report, for the first time, the use of poly[ferrocene]-1H-pyrrole, a patented poly-pyrrole derivative synthesized at Technobiochip, to create, using Langmuir-Shaeffer thin film deposition method, an interfacial chemistry on the crystal quartz golden surface to firmly stabilize antibody molecules. Coated quartzes were used to perform a classical antigen-antibody binding reaction on Technobiochip's μLibra 3.1 (Quartz Crystal Microbalance). Our results demonstrate that the antibody binding on coated quartzes was extremely increased (70%) over that shown by bare quartzes. In addition, the antigen-antibody interaction was also significantly improved (30%).