Ultra-low-level infra-red laser light irradiation on cultured fibroblasts: effects on mitochondrial and structural proteins

摘要

Red light has a great impact on living organisms also at cell level. We investigated the effect of photostimulation in the red-to-near infrared spectrum wavelength delivered by a laser device at extremely low level on cultured murine fibroblasts. We showed that three days of pulsed exposure cause the increase of cytochrome C staining in mitochondria. Membrane-bound actin immunostaining decreases under red-light exposure, and cell shape is modified. The body/elongation ratio decreases, suggesting that fibroblasts acquire a more dynamic morphology. These data indicated that even extremely low levels of pulsed red light affects living cells.