Effect of low temperature on the protein content of the thylakoid lumen of Arabidopsis thaliana

摘要

The completion of the Arabidopsis thaliana genome-sequencing project by the end of 2000 [The Arabidopsis Genome Initiative, 2000] started a new era in plant research. To apply the knowledge of the Arabidopsis genome to an investigation into the chloroplast lumen, we designed a purification method for the lumenal proteins from Arabidopsis chloroplasts and studied them systematically by proteomics [Schubert et al., 2001]. Based on these and other recent results, a new picture of the thylakoid lumen of higher plant chloroplasts is emerging. The lumenal compartment is no longer only a space for accumulating protons and ensuring charge balance during ATP production, but seems to play an important role in regulating the stability, function and assemblyof the different photosynthetic complexes. In this study we applied our 2D-maps to analyse, if the lumen proteome changes upon cold treatment. Our preliminary data indicate that the proteins expressed in the thylakoid lumen indeed changed when Arabidopsis plants were grown in low temperature.