SELECTIVE MOLECULAR PROBES FOR DETECTING AROMATIC-COMPOUNDS DEGRADING BACTERIA IN ENVIRONMENTAL SAMPLES

摘要

A set of ERIC primers was assayed for the detection of some aromatic compound-degrading bacteria in environmental samples. Pseudomonas GCH1 strain was used in contaminated soil bioremediation, and amplification by PCR using the DNA isolated from soil gave a specific DNA band pattern corresponding to the GCH1 strain. Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data of homologous genes, two different oligonucleotides were designed which have been assayed for detecting 3,4-DHPA related aromatic-compounds degrading bacteria in soil samples. The results Indicated that these molecular techniques were highly specific and may represent a powerful tool for efficient remediation of xenobiotics pollutants by natural microbial communities.