Nano Size Biological Clock Capsulated by Lipid Layer: Reconstitution of biological clock into phospholipid-coated microdroplets

摘要

We propose a nano size clock, capsulated into micro size liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ≈24-hour (circadian) period. In cyanobacteria, circadian clock was reconstituted by only three proteins. We reconstitute these proteins and adenosine triphosphate (ATP; energy of clock) into lipid layer and confirmed the clock function. This system will reveal more detail about biological clock and bring application possibility.