Identification and expression of human papilloma virus type 16 L1 Gene in E.coli

摘要

It has been confirmed that the infection of Human papilloma virus type 16 (HPV16) was highly related with the occurrance of women cervical carcinoma. And it has great practical significance to explore HPV16 genetic vaccine that can prevent the infection of the same type virus. Methods PCR method was applied to amplify L1 gene fragment from HPV16L1 Chinese isolate. The recombinant pGEX4T-1 vector. We transformated the recombinant plasmid into E.coli (BL21) and the L1 protein was indcued with IPTG. The L1 protein was identified by means of SDS-PAGE and Western blot. Results The recombinant pGEX4T-1-HPV16L1 was successfully identified and L1 protein was expressed with high quantity. Conclusions pGEX4T-HPV16L1 could express L1 protein with high production that could make good basis for further research.