Structure and Function of Two Distinct Types of Prostaglandin D Synthase

摘要

Prostaglandin (PG) D synthase (PGDS, EC 5.3.99.2) catalyzes the isomerization of PGH_2, a common precursor of various prostanoids, to produce PGD_2 in the presence of sulfhydryl compounds. PGD_2 induces sleep [1], regulates nociception, inhibits platelet aggregation, acts as an allergic mediator [_2], and is further converted to 9a, 11 p-PGF_2 or the J series of PGs, such as PGJ_2, DELTA~(12)-PGJ_2, and 15-deoxy-DELTA~(12)-PGJ_2. 15-Deoxy-DELTA~(12,14)-PGJ_2 is a ligand for PPAR(gamma), a nuclear receptor involved in differentiation of adipocytes, macrophages, and monocytes. Two distinct types of PGDS have been identified; one is the lipocalin-type enzyme (L-PGDS); and the other, the hematopoietic enzyme (H-PGDS). For each enzyme, we isolated the cDNA and the gene, determined the X-ray crystallographic structure, tissue distribution profile, and the cellular localization in several animal species, and generated gene-knockout (KO) and human enzyme-overexpressing transgenic (TG) mice. Although L-PGDS and H-PGDS catalyze the same reaction, they are quite different from each other in terms of their catalytic properties, amino acid sequence, tertiary structure, evolutional origin, gene structure, chromosomal localization, cellular localization, tissue distribution, and also functional relevance, as summarized in Table 1. Therefore, we consider that L-PGDS and H-PGDS are a novel example of functional convergence. We have recently reviewed a part of these findings elsewhere [3,4].