Configuration of a simple dialysis membrane bioreactor suitable for large-scale application

摘要

We have developed a prototype dialysis reactor of IOL working volume, which combines the advantages of batch and of continuous fermentation processes and allows further calability. Batch fermentations with and without an integrated dialysis membrane were compared. We could demonstrate considerable improvements regarding cell densities, cell viability and product titer by the integration of a dialysis membrane. Evaluation of the transport of lactate and glucose across the dialysis membrane indicated an efficient removal of waste products and a sufficient supply of nutrients. The system has a high potential for industrial application.