Industrial dye decolorization by laccases from ligninolytic fungi

摘要

White-rot fungi were studied for the decolorization of twenty-three industrial dyes. Laccase, manganese peroxidase, lignin peroxidase and aryl alcohol oxidase activities were determined in crude extracts from solid state cultures of sixteen different fungal strains grown on whole oats. All Pleurotus ostreatus strains exhibited high laccase and manganese peroxidase activity but highest laccase volumetric activity was found in Trametes hispida. Only laccase activity correlated with the decolorization activity of the crude extracts. Two laccase isoenzymes from Trametes hispida were purified and their decolorization activity was characterized.