Cloning an Phylogenetic Analysis of Chitin Synthase Coding Genes of Penicillium chrysogenum

摘要

DNA fragments homologous to chitin synthase were amplified from the genomic DNA fo penicillium chrysognum by PCR. Clonign and sequencing of the PCR-amplified fragments led to the identification of four different genes, designated PcCHSI, PcCHS2, PcCHS3, and PcCHS4. By comparison of the deduced amino acid sequences, PCCHSI was identified as a gene for class I chitin synthase, PcCHS2 and PcCHS3 were for class II, and PcCHS4 was for class III. For the phylogenetic study fo P.chrysogenum, the blast P similarity search and the bootstrap analysis fo the deduced amino acid sequences of the highly conserved regiion of chitin synthase genes were performed. The analysis of amino acid sequences implicateda close evoletionary relationship of penicillium to ascomycetours fungi, especially to genus aspergillus. The results from bootstrap analysis fo the chitin synthase also supported the usefulness of the chitin synthase for phylogenetic study of filamentous fungi.