A novel hypothesis-testing technique for resolving overlapping peaks in DNA sequencing

摘要

The accuracy of determining the Deoxyribo Nucleic Acid (DNA) sequence is limited by overlapping peaks of the same base-type (A,C,G or T). Commonly used iterative method to resolve these peaks tends to generate false peaks and has limitedresolution. This paper presents a novel hypothesis-testing method, which uses a statistical model of DNA time series, to estimate position and amplitude of the overlapping peaks. The new method has better resolution than the iterative method and does notgenerate false peaks.