In vivo osteogenic potent of cultured bone/hydroxyapatite construct: a comparison with that of marrow cell/hydroxyapatite composite

摘要

The ideal bone graft is autogenous bone graft. However, it is not available in quantities needed for bone reconstruction and involves invasion of healthy tissue. To overcome this problem, we employed dexamethasone-treated marrow cell culture technology developed by Maniatopoulos et al., which produces bone tissue in vitro. We previously reported that the osteogenic construct, which combines bone formed in vitro and porous hydroxyapatite (HA), has high osteogenic potential when grafted in vivo. In the present study, we compared the in vivo osteogenic capacity of bone/HA construct with marrow cell/HA composite. Results obtained suggest that the cultured bone /HA graft construct procedure is an excellent method for obtaining more bones in a shorter time compared with the cell/HA composite.