The Effect of Interleukin-6 and Leukemia Inhibitory Factor on Hybridoma Cells

摘要

In mammalian cell culture, adaptation to serum-free medium is very important. We found that such adaptation influenced the sensitivity of hybridoma cells to cytokines. Both interleukin-6 (IL-6) and leukemia inhibitory factor (LIF) are known as mitogens for hybridomas. Before adaptation, in the presence of IL-6, the proliferation of parental 2E3 hybridoma cells was increased, as well as in the presence of LIF. After adaptation to serum-free medium, the response of the hybridoma cells to the cytokineswas transformed. In the presence of IL-6 and LIF, the proliferation of 2E3-0 cells, sub-line adapted to serum-free medium, was suppressed. Despite this, DNA fragments were not observed in 2E3-0 cells treated with IL-6 and LIF, suggesting that neither cytokine induces apoptosis.