Independent component analysis (ICA) was used to separate action potential trains recorded with voltage-sensitive dyes in the isolated brain preparation of the sealsium Tritonia. It was assumed that the mebrane potential of each neuron was an approximately independent source and the detectors (photodiodes) recorded linear mixtures of sources. These assumptions appear to be resonable as ICA outperformed existing methos for separating spike trains in optical recording data. Spike trains, artifiacts, and noise were assigned to different output channels (indepenent components). The ICA analysis method was also less labor intensive than other methods.
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