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Spark source infrared fluorometry

机译:火花源红外荧光法

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摘要

The application of our development of 750 nm excitation from an argon-hydrogen filled spark source to time-resolved fluorescence probe studies of lipid membranes and inverse micelles is reported. The laser dye IR-140 was studied using the single- photon counting technique both in a lipid membrane of L-$alpha dipalmitoylphosphatidylcholine (DPPC) and in sodium sulfosuccinic acid bis (2-ethylhexyl) ester (AOT) in iso-octane. In DPPC a dramatic change in the fluorescence behavior of IR-140 is observed between the gel and liquid crystalline phases. In inverse micelles of AOT an increase in the intensity of the peak fluorescence emission and a decrease in fluorescence lifetime is noticed on increasing the water content.
机译:据报道,从氩气填充火花源到脂质膜和逆胶束的氩气填充火花源的750nm激发的应用。使用L- $α二硫代钯(DPPC)的脂质膜和在异辛烷中的磺基碱酸双(2-乙基己基)酯(AOT)中的单光子计数技术研究了激光染料IR-140。在DPPC中,在凝胶和液晶相之间观察IR-140的荧光行为的显着变化。在AOT的逆胶束上,在增加水含量时,注意到峰荧光发射强度的增加和荧光寿命的降低。

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