Alignment and Deformation of MC3T3-E1 Cultured on Micro Striped Pattern after Stimulation of Tangential Force Field

摘要

The effect of hysteresis of the tangential force field on the single cell cultured at the surface of the scaffold with the micro striped pattern has been studied in vitro. The lines of parallel micro quadrangular ridges (0.7 um height, 3 urn width, and 3 um interval) were made on the surface of the scaffold plate by the photolithography technique to control the orientation of each cell. Variation was made about the angle between the longitudinal direction of the ridge and the direction of the centrifugal force field: 0 degree, 45 degrees, and 90 degrees. To apply the tangential force field (50 G, 100 G) on the surface of the scaffold, the plate was set in the tube in a conventional centrifugal machine placed in an incubator. After the centrifugation for 5 hours, the contour of each cell at the time lapse microscope images was traced for 24 hours in the incubator, and the angle between the longitudinal axis of the cell and the direction of the centrifugation was analyzed. The experimental results show that the MC3T3-E1 (mouse osteoblast precursor cell) elongates to the longitudinal direction of the striped pattern by the hysteresis of the exposure to the tangential force field more frequently at 100 G than at 50 G