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Analysis of microbiologically influenced corrosion of carbon steel in soil

机译:土壤中碳钢腐蚀的微生物学影响分析

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Recently, deteriorated tubing materials buried in the soil are problematic issues. They are thoughtto be caused not only by physicochemical factors but also by biological factors such as microbesin the surrounding soil. Therefore, it is necessary to clarify the relationship between the metalcorrosion and microbial consortia in the soil.In this study, carbon steel coupons (25x10x0.5 mm) were buried in the soil taken from a forest. Allof them were conducted by using the 90 mm petri dishes. The water contents were adjusted at 0,50, 75, 100%, respectively. These samples were incubated aerobically or anaerobically for severalmonths. During the incubation, from the corroded products attached to the coupons and adjacentsoil, bacterial genome was extracted and purified by the beads-beating method and phenolchloroformmethod. 16S rRNA genes of purified genome were amplified to analyze the microbialconsortia. Statistical analysis was done by QIIME software. The corrosion rate and the corrodedsurface were also measured by weight loss and laser displacement meter, respectively.The average corrosion rate under aerobic condition was much higher than that under anaerobiccondition except for 0% water content. Under 0% water content condition, there was no significantdifference between aerobic and anaerobic conditions. Especially, when the water content was 50%and/or 75% under aerobic condition, the high corrosion rates were observed. Moreover, severepitting corrosion occurred. On the other hand, under anaerobic conditions, the water content didnot strongly affect the corrosion rate and the pitting corrosion was not observed. Concerning themicrobial consortia in the corrosion product and soil, there were big differences between aerobiccondition and anaerobic condition. However, the specific species such as sulfate-reducingbacteria that might contribute the microbiologically influence carrion has not been identified. In thefurther study, more detail statistical analysis of microbial consortia is needed.
机译:最近,埋藏在土壤中的恶化的管材是有问题的问题。他们是想法的 不仅由物理化学因素而造成的,而且是微生物等生物因素 在周围的土壤中。因此,有必要阐明金属之间的关系 土壤中的腐蚀和微生物聚焦。 在本研究中,碳钢券(25x10x0.5 mm)埋在森林中的土壤中。全部 它们是通过使用90毫米培养皿进行的。将水含量调节为0, 分别为50,75,100%。将这些样品有氧或厌氧地孵育几个 几个月。在孵育期间,从附着在优惠券和邻近的腐蚀产品 用珠子串联方法提取和纯化土壤,细菌基因组,纯化苯酚 方法。扩增纯化基因组的16S rRNA基因以分析微生物 联盟。统计分析由奇维软件完成。腐蚀速率和腐蚀 表面也分别由减肥和激光置换仪测量。 有氧病症下的平均腐蚀速率远高于厌氧下的腐蚀速率 条件除外0%含水量。在0%的水含量条件下,没有显着的 有氧和厌氧条件之间的差异。特别是,当水含量为50%时 和/或在有氧病症下75%,观察到高腐蚀速率。而且,严重 发生腐蚀。另一方面,在厌氧条件下,水含量为 没有强烈影响腐蚀速率,未观察到蚀腐蚀。关于这一点 微生物联盟在腐蚀产品和土壤中,有氧之间存在巨大差异 病情和厌氧条件。但是,硫酸盐降低等特异性物种 尚未确定可能有助于微生物源影响腐肉的细菌。在里面 进一步的研究,需要更详细的微生物组成的统计分析。

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