We propose a new methodology for isolation of a microbe by theoptical radiation pressure and dielectrophoretic force under themicroscope. Our methodology is based on the fact that an arbitrarysingle microbe can be isolated speedily in a microchannel, even thoughthere are a large number of these microbes in a solution. We show thatonce the target microbe is trapped at the focal point of the lasermanipulator, we can easily realize the non-contact exclusion of a largenumber of excess microbes immediately by controlling the electric field,while keeping the target at the focal point. To improve the operabilityof the micromanipulation in solution and to realize high-speedseparation, the technique of sequential, precise and non-invasivehandling is necessary. We propose a new teleoperation method usingbilateral control to improve the operability of micromanipulation. Ourmethod can be extremely useful in the pure cultivation of the targetedmicrobe and will be a promising method for biologists in discovering anew microbe
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