Dual-channel structured illumination microscopy setup based on Digital Micromirror Device

摘要

Structured illumination microscopy (SIM) possesses higher imaging speed among SR imaging technologies, beneficial to living cell research. With the increase of application requirement, SIM has met challenges in multi-color imaging. The current multi-color SIM imaging is generally time-lapse imaging, making it unsuitable for living cell observation. Thus, we proposed a dual-channel SIM setup based on Digital Micromirror Device (DMD). DMD enables the same modulation of excitation lights simultaneously, and opto-splitter achieves dual-channel images collection simultaneously. We verified our dual-color SIM setup in experiment and its imaging speed is no longer limited by the wavelength. Compared to other dual-color SIM imaging methods, our method doubles the multi-color imaging speed, making it prospective to fast, multicolor SR imaging in the future study of living cells.