Method Validation for Analysis of Parabens and Antimicrobials in Human Hair

摘要

Background/Aim Humans are constantly exposed to new chemicals. Among them, harmful substances are also included. EDCs affect human hormones and interfere with the endocrine system. Representative EDCs include parabens and antimicrobials. Samples of blood, serum, and urine are typically used to assessment of human exposure to EDCs. These samples are not adequate to reflect long-term exposure. Hair sample has been used for chronic and past exposure assessments in drug, doping and forensic studies. In this study, we investigate the possibility of biomonitoring through hair, and established pretreatment and analytical method for EDCs in hair. Methods A recovery test was conducted to establish six parabens and two antibacterial agents in hair. Hair samples (0.05g) were pretreated through LLE extraction with methanol (2mL), after added IS and STD. Quantitative analysis was performed using UPLC-MS/MS. Intra (n=7) and Inter (3day) validation were conducted to verify the validity of pretreatment and analytical method. Results As a result of the validation, accuracy were 34.4 ～ 110.4 % (inter) and 68.9 ～ 112.9 % (intra). Precision were 2.1 ～ 36.9 % (inter) and 2.1 ～ 21.7% (intra), and LOD were 0.108 (benzylparaben)～ 0.864(triclosan) ng/ml respectively. Conclusions EDCs biomonitoring by hair analysis will help us to overcome the difficulties in sampling from infants and long-term exposure assessment.