Fast imaging of cardiomyocyte dynamics alterations after drug treatment utilizing quantitative phase digital holographic microscopy

摘要

Digital holographic microscopy (DHM) is an interferometry-based variant of quantitative phase microscopy (QPM) thatcan be integrated modular into various common microscopes for label-free imaging of fast cellular morphology changesin a biomedical laboratory environment. We have utilized a fiber optics-based off-axis DHM concept to monitor the dynamicsof beating cardiomyocytes after drug treatment. Our results show that local height changes due to cellular contractionscan be detected spatially resolved at the subcellular level. Moreover, we demonstrate that a temporal resolution inthe millisecond range is sufficient to detect drug induced increases and decreases of the beating rate.