Toward Point-of-care Uropathogen Detection Using SERS Active Filters

摘要

150 million people worldwide suffer one or more urinary tract infections (UTIs) annually. UTIs are a significant healthburden: societal costs of UTI exceed $3.5 billion in the U.S. alone; 5% of sepsis cases arise from a urinary source; andUTIs are a prominent contributor toward antimicrobial resistance (AMR). Current diagnostic frameworks exacerbate thisburden by providing inaccurate and delayed diagnosis. Rapid point-of-care bacterial identification will allow for earlyprecision treatment, fundamentally altering the UTI paradigm.Raman spectroscopy has a proven ability to provide rapid bacterial identification but is limited by weak bacterial signaland a susceptibility to background fluorescence. These limitations may be overcome using surface enhanced Ramanspectroscopy (SERS), provided close and consistent application of bacteria to the SERS-active surface can be achieved.Physical filtration provides a means of capturing uropathogens, separating them from the background solution and actingas SERS-active surface.This work demonstrates that filters can provide a means of aggregating bacteria, thereby allowing subsequent enhancementof the acquired Raman signal using metallic nanoparticles. 60 bacterial suspensions of common uropathogens were vacuumfiltered onto commercial polyvinylidene fluoride membrane filters and Raman signals were enhanced by the addition ofsilver nanoparticles directly onto the filter surface. SERS spectra were acquired using a commercial Raman spectrometer(Ocean Optics, Inc.). Principal Component – Linear Discriminant Analysis provided discrimination of infected fromcontrol samples (accuracy: 88.75%, 95% CI: 79.22-94.59%, p-value <0.05). Amongst infected samples uropathogens wereclassified with 80% accuracy.This study has demonstrated that combining Raman spectroscopy with membrane filtration and SERS can provideidentification of infected samples and rapid bacterial classification.