Characterization of single-molecule dwell times beyond photobleaching

摘要

In this work we report a single molecule imaging method to spread the photon budget of a fluorophore overextended time periods by inserting a non-illuminating interval between consecutive frames. While photobleaching resultsin loss of fluorescent signals similar to the detachment of a fluorescently labeled biomolecules, we introduced this methodto characterize the dwell times of single molecules.