Quantitative phase imaging camera with a weak diffuser based on the transport of intensity equation

摘要

We present an efficient quantitative phase imaging camera with a weak diffuser (QPICWD) based on the transportof intensity equation (TIE). The compact QPICWD measures object induced phase delay under low-coherencequasi-monochromatic illumination via examining the deformation of the speckle intensity pattern. Analysing thespeckle deformation with an ensemble average of the geometric ow, we can achieve the high-resolution distortioneld by the TIE. We present some applications for the proposed design involving nondestructive optical testingof microlens array with nanometric thickness and imaging of xed and live unstained HeLa cells. Since thedesigned QPICWD needs no modication of the common bright-eld microscope or additional accessories, itmay advance QPI as a widely useful tool for biological analysis at subcellular levels.