Microspectrometry-FTIR based Glucose and Fructose Biosensor with Pseudo-Continuous Flow

摘要

In this work, we present a new microspectrometry FTIR-based biosensor for the analysis of sugar based solutionsusing various solvent and various types of sample handling support (SHS). We used methanol as solvent becauseit does not interact with infrared (IR) beam. In addition, it ensures a rapid evaporation in order to form athin layer of targeted molecules on the conventional SHS. The later consists of a total reective mirror (TRM).Because of methanol toxicity, we developed a new setup for aqueous samples analysis based on accelerated solventevaporation. The achieved limit of detection (LOD) using the developed biosensor was 3 mM for both, Glucoseand Fructose molecules.