Visible light optical coherence microscopy for quantitative imaging of human skin in vivo

摘要

Recent development of visible light Optical Coherence Microscopy (i.e. Optical Coherence Tomography employinghigh numerical aperture Objective) has pushed the axial/transverse resolution limit to < 2 μm within tissues.Here, a fiber-based visible light Fourieer domain (FD) Optical Coherence Microscopy system is presented, employinga 10× water immersion Objective to achieve higher transverse resolution and reduced specular reectionwhen imaging human skin in vivo, using ~1-2 mW of incident power. Though with limited penetration depth,we demonstrated its application for in vivo imaging of human skin, and further measured the thicknesses ofepidermis and dermo-epidermal junction. Furthermore, the total attenuation coefficient of the skin tissues wasalso estimated across epidermis and dermis.