Graphene oxide based FRET probe for mast cell degranulation

摘要

Degranulation in mast cell is usually characterized by the release of tryptase. We developed a fluorescence resonanceenergy transfer (FRET) probe based on graphene oxide (GO) to detect tryptase released from mast cells. The GO basedFRET probe is composed of GO and a self-assembled complex of tryptase-specific recognition peptide chains labeledwith isothiocyanate fluorescein. The fluorescence intensity around the mast cells increased when the mast cells werestimulated with C48/80, a kind of reagent promoting degranulation. The fluorescence distribution is inhomogenous. Thefluorescence intensity was dependent on the concentration of C48/80 and the stimulation time. These results demonstratethat GO-base FRET probe could be used to study degranulation in mast cells.