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Light-sample interaction in microsphere enhanced 2D superresolution imaging

机译:微球增强2D超分辨率成像中的光样本相互作用

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We simulate the image generated by a microsphere residing in contact on top of an exposed Blu-ray disk surface, whenobserved by a conventional microscope objective. While microsphere lenses have been used to focus light beyond thediffraction limit and to produce super-resolution images, the nature of the light-sample interaction is still under debate.Simulations in related articles predict the characteristics of the photonic nanojet (PNJ) formed by the microsphere, but sofar, no data has been published on the image formation in the far-field. For our simulations, we use the open source packageAngora and the commercial software RSoft FullWave. Both packages implement the Finite Difference Time Domain(FDTD) approach. Angora permits us to accurately simulate microscope imaging at the diffraction limit. The RSoftFullWave is able to record the steady-state complex electrical and magnetic fields for multiple wavelengths inside thesimulation domain. A microsphere is simulated residing on top of a dielectric substrate featuring sub-wavelength surfacefeatures. The scattered light is recorded at the edges of the simulation domain and is then used in the near-field to far-fieldtransformation. The light in the far field is then refocused using an idealized objective model, to give us the simulatedmicroscope image. Comparisons between the simulated image and experimentally acquired microscope images verify theaccuracy of our model, whereas the simulation data predicts the interaction between the PNJ and the imaged sample. Thisallows us to isolate and quantify the near-field patterns of light that enable super-resolution imaging, which is importantwhen developing new micro-optical focusing structures.
机译:我们模拟了由微球在暴露的蓝光磁盘表面顶部接触时生成的图像,当 用常规的显微镜物镜观察。虽然微球透镜已用于将光线聚焦到 衍射极限和产生超分辨率图像,光-样品相互作用的性质仍在争论中。 相关文章中的模拟预测了由微球形成的光子纳米射流(PNJ)的特性,但是 到目前为止,尚未发布有关远场成像的数据。对于我们的模拟,我们使用开源软件包 Angora和商业软件RSoft FullWave。这两个软件包都实现了时差有限域 (FDTD)方法。安哥拉使我们能够在衍射极限下精确模拟显微镜成像。 RSoft FullWave能够记录内部多个波长的稳态复杂电场和磁场 模拟域。模拟了一个微球,该球位于具有亚波长表面的介电基板上 特征。散射光记录在模拟域的边缘,然后用于近场到远场 转型。然后,使用理想化的物镜模型将远场中的光重新聚焦,从而为我们提供模拟的 显微镜图像。模拟图像与实验获得的显微镜图像之间的比较验证了 我们模型的精度,而模拟数据预测PNJ和成像的样本之间的相互作用。这 使我们能够隔离和量化能够实现超分辨率成像的光的近场模式,这一点很重要 在开发新的微光学聚焦结构时。

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