Three-dimensional tracking of moving Volvox by parallel phase-shifting digital holographic microscope

摘要

We report the three-dimensional (3D) trajectory of a Volvox moving in water was recorded by parallel phase-shifting digital holographic microscope providing 10X magnification. The recording frame rate, the shutter speed, and the total recording time were 1000 fps, 0.25 ms, and 2.1 s, respectively. In the reconstructed phase image of the Volvox, the shape of the Volvox is regarded as a circle. The lateral coordinates of the Volvox were determined as the center of the circle. The depth coordinates of the Volvox were determined as the propagation distance where the edge of the Volvox in the reconstructed amplitude image was clearest while the propagation distance was varied. We successfully demonstrated the 3D tracking of curvedly moving Volvox.