Digital microfluidic biochips (DMFBs) have become popular in the healthcare industry recently because of its lowcost, high-throughput, and portability. Users can execute the experiments on biochips with high resolution, and the biochips market therefore grows significantly. However, malicious attackers exploit Intellectual Property (IP) piracy and Trojan attacks to gain illegal profits. The conventional approaches present defense mechanisms that target either IP piracy or Trojan attacks. In practical, DMFBs may suffer from the threat of being attacked by these two attacks at the same time. This paper presents a comprehensive security system to protect DMFBs from IP piracy and Trojan attacks. We propose an authentication mechanism to protect IP and detect errors caused by Trojans with CCD cameras. By our security system, we could generate secret keys for authentication and determine whether the bioassay is under the IP piracy and Trojan attacks. Experimental results demonstrate the efficacy of our security system without overhead of the bioassay completion time.
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