Cultivation of Aurantiochytrium sp. L3W in Food-processing Wastewater and Possible Utilization of Biomass for Fish Feed Additive

摘要

Polyunsaturated fatty acids (PUFAs) contained in fish oil essential for growth of fish, whereas the demand for fish oil is increasing because of the advantages of PUFAs in human health preservation. To realize sustainable aquaculture, alternatives of fish oil are necessary. Here, we propose a scenario where PUFA-producing Aurantiochytrium is cultivated using food processing wastewater to achieve both utilization of the unused resource and production of alternative fish oil. In this study, two wastewater samples discharged from bean-boiling (BB) and miso production (MP) processes in a miso processing factory were applied for cultivation Aurantiochytrium sp. strain L3W under sterilized and unsterilized conditions. The strain L3 W grew in both MP and BB wastewater samples, while the sterilized condition demonstrated better growth. Comparing carbon and nitrogen consumption, the removal efficiencies of both constituent of the BB sample were better than that of MP one. Finally, the analysis of the resultant biomass revealed that PUFAs were produced from both BB and MP samples by the strain L3W.