Novel fluorescent oxides provide insight into dynamics of nanoparticle- mediated drug uptake from gastro-intestinal tract

摘要

In recent years nanotechnology gathered much attention due to promising applications in biomedicine. Using nanoparticles as drug carriers could allow for more effective and efficient therapy in treating cancer or neurological diseases. This is due to their unique properties such as enhancement of drug bioavailability or the ability to protect the drug from degradation. In this study we performed in vivo (BALB-c mice) and in vitro (Caco-2 cell line) experiments with Y_2O_3Tblectin conjugates as well as pure lectin to characterize the dynamics of nanoparticles mediated drug uptake from gastro-intestinal tract. Mice were given 0.3ml of Y_2O_3:Tb_lectin conjugates or pure lectin suspension and were sacrificed after 3h, 24h and 1 week (Y_2O_3b:Tb_lectin conjugates) or 3h and 24h (pure lectin). Cell cultures were incubated for 24h with increasing concentration (0.001mg/ml; 0.01mg/ml; 0.1mg/ml; 1mg/ml) of Y_2O_3:Tb_lectin or pure lectin. After analysing gathered data we concluded that our nanoparticles successfully conjugated with lectin and allowed for its transport through physiological barriers. NPs_Lectin conjugates undergo absorption, distribution and redistribution similarly as free nanoparticles do, although it decreased the efficiency of absorption compared to free nanoparticles. Lastly after reaching the tissue conjugates dissolved leading to lectin deposition in the tissue.