Electrospinning of collagen with nanocapsules of PLGA for delivery of paclitaxel in drug -eluting stents

摘要

This work develops a new delivery system trying to understand better, the factors that influences the drug release. This system use nanofiber produced by electrospinning technic and bioabsorbable polymeric nanocapsules produced by the nanoprecipitation processes in a recirculated system develop in the research group. For this work, we used an electrospinning equipment built at the university, in which you can control the process parameters such as voltage, deposition rate, collector distance, speed and direction of rotation of the collector, to control the morphology and diameter of the nanofibers. The final electrospinning condition to obtain nanofiber of collagen (Sigma-Aldrich) with 99 ± 26 nm was 18 V, collector distance of 14cm, flow rate of 0.1mL/h and polymer concentration was 25% W/V in acetic acid and distilled water 9:1. On the other hand, we encapsulated Paclitaxel in bioabsorbible polymeric nanocapsules of PLGA (Resomer 752 H, Evonik) produced in a recirculated system design in the university. The conditions used to encapsulate the paclitaxel was 4mg/mL of polymer concertation, 20% of drug respect to dry polymer, 162 mL/min of flow rate in the recirculating system, 0.25% of Pluronic F-127 (Sigma-Aldrich) as surfactant in the aqueous phase and 2:1 ratio between aqueous phase and organic phase.