Optimizing media for perfusion combining predictive scale-down models and multivariate approaches

摘要

Perfusion medium development and optimization offers great performance increases for perfusion processes. We present several aspects of the capabilities we nave developed recently to support both customized perfusion medium optimization as well as catalog products. Critical aspects indude a task specific medium panel to start development, various scale-down models (SDM) to achieve representative results during optimization in different phases of development, and multivariate tools to facilitate component optimization. Our example shows how media can be designed and optimized for different target outcomes. Using these capacities we developed the first-of-its-kind EXCEL®. AdvancedTM HD Perfusion medium, which allows for high ceH density perfusion at lowest possible Derfusion rates.