The liquid stillage contained reducing sugars (fructose and glucose) and volatile fatty acids including acetic acid, propionic acid and butyric acid. 0.5 g/L of reducing sugar was found in raw stillage. Thereafter, ethanol was harvested and the stillage after ethanol recovery was utilized for polyhydroxybutyrate (PHB) production. The effect of stillage concentration, nitrogen source, temperature, agitation speed and initial pH were studied. The optimum condition for PHB production containing 50% of stillage with 5 g/L yeast extract as nitrogen source. The cultivation was incubated at 30°C under the agitation speed of 150 rpm and pH 7. The highest biomass (10.50±0.141 g/L) and PHB (5.75 g/L, 86.50±6.236% of dry cell weight, DCW) were obtained under optimum conditions. The PHB was further utilized for hydroxybutyrate methyl ester (HBME) production. The partial properties of HBME were studied.
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机译：液态釜馏物包含还原糖（果糖和葡萄糖）和挥发性脂肪酸，包括乙酸，丙酸和丁酸。在未加工的蒸馏物中发现了0.5 g / L的还原糖。此后，收获乙醇并将乙醇回收后的釜馏物用于生产聚羟基丁酸酯（PHB）。研究了馏分浓度，氮源，温度，搅拌速度和初始pH的影响。 PHB生产的最佳条件是含有50％的蒸馏物，并以5 g / L酵母提取物作为氮源。将培养物在30℃，150 rpm的搅拌速度和pH值为7的条件下孵育。最高生物量（10.50±0.141 g / L）和PHB（5.75 g / L，干细胞重量的86.50±6.236％，DCW）为在最佳条件下获得。 PHB还用于羟基丁酸酯（HBME）的生产。研究了HBME的部分性质。
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