Action of newly developed tetrazolato-bridged dinuclear platinum(II) complexe as the candidate of effective antitumor drug on the structure of large DNA

摘要

We report the action of tetrazolato-bridged dinuclear platinum(II) complex, 5-H-Y (2+), and SPD (3+) on the higher-order and secondary structure of DNA through the observations with fluorescence microscopy and CD measurements. From the measurement on the higher-order structure of a giant DNA in solution with fluorescence microscopy, it was found that 5-H-Y (2+) causes shrinking of DNA above 10 μM. On the contrary, SPD (3+) showed negligible change on the higher-order structure of DNA up to 30 μM. From circular dichroism (CD) measurements, it becomes clear that 5-H-Y (2+) induces the transition of the secondary structure of DNA from B-form to C-form.