The measurement of depth of field in microscope system based on image clarity evaluation

摘要

In order to realize the digital measurement of the depth of field in microscope systems, a method of measuring the depth of field in microscope systems based on image clarity evaluation is proposed. The basic principle of the method is: pay attention to the image clarity evaluation based on wavelet transform when changing the distance between the microscope lens and the sample. When the measurement is reduced to the threshold value, the image returns blurred at once. Then the distance of the movement between the microscope and the sample is the result. The method is tested in many different microscope systems. The results show that the method is feasible, uses less time and has better robustness. The accuracy can reach more than 95.34%, proving that image processing technology can be applied in the area of the measurement of the depth of field.