Stigmasterol from the Flowers of Trollius chinensis

摘要

After being extracted with 75 % ethanol, Trollius chinensis extract was obtained by evaporation of its supernatant under vacuum conditions and dissolved with water, followed by extraction with petroleum ether. The petroleum ether part was collected and subjected to silica gel column chromatography and separated individual. Components were further purified by several rounds of silica gel column, followed by recrystallization with methanol. In total, three compounds were isolated from the air-dried flowers of Trollius chinensis and identified as β-sitosterol, stigmasterol and hellebore acid based on spectral analysis, NMR experimentation. Among these three compounds, stigmasterol was found for the first time in Trollius chinensis.