Dynamically constrained pipeline for tracking neural progenitor cells

摘要

Large scale in vitro cell growth experiments require automated segmentation and tracking methods to construct cell lineages in order to aid cell biologists in further analysis. Flexible segmentation algorithms that easily adapt to the specific type of problem at hand and directly applicable tracking methods are fundamental building blocks of setting up multi purpose pipelines. Segmentation by discriminative dictionary learning and a graph formulated tracking method constraining the allowed topology changes are combined here to accommodate for highly irregular cell shapes and movement patterns. A mitosis detector constructed from empirical observations of cells in a pre-mitotic state interacts with the graph formulation to dynamically allow for cell mitosis when appropriate. Track consistency is ensured by introducing pragmatic constraints and the notion of blob states. We validate the proposed pipeline by tracking pig neural progenitor cells through a time lapse experiment consisting of 825 images collected over 69 hours. Each step of the tracking pipeline is validated separately by comparison with manual annotations. The number of tracked cells increase from approximately 350 to 650 during the time period.