Study of DNA Fingerprint of Pine Wood Nematode Based on Terahertz Spectroscopic Technology at 0-10 THz

摘要

In this paper, we investigate the fingerprint characteristics of harmful Bursaphe-lenchus xylophilus (Bx) nematode and harmless Bursaphelenchus mucronatus (Bm) nematode by applying terahertz spectroscopic techniques. We measure and analyze the transmission features of the deoxyribose nucleic acid (DNA) of the Bx and the Bm samples, and their corresponding polymerase chain reaction (PCR) amplification segments of the DNA molecules by using terahertz domain spectroscopy (THz-TDS) and Fourier transform infrared spectroscopy (FTIR). According to the low frequency measurement below 2.5 THz by THz-TDS, the calculated relative refractive index of the Bm-PCR segment is found to be much smaller compared with other three samples. The higher frequency characteristics of the four samples measured by the FTIR spectroscopy shows similar absorption peaks, except that the Bm-DNA provides a smooth differential feature of absorption at 5.46 THz. These measurement results indicate the Bm samples including DNA and PCR segment have different spectroscopic behaviors from the Bx samples. It demonstrates that the terahertz spectroscopic technique is a useful method to distinguish the Bx and the Bm's DNA and PCR segments by locating the absorption frequency.