3-D motion-picture recording by parallel phase-shifting digital holographic microscopy

摘要

Three-dimensional (3-D) motion-picture recording by parallel phase-shifting digital holographic microscopy that has the ability of instantaneous 3-D recording of dynamic phenomena in the microscopic field of view is presented. Parallel phase-shifting digital holography is a scheme to record multiple phase-shifted holograms with a single-shot exposure, and to achieve 3-D motion-picture recording of objects with high accuracy and wide 3-D area, based on space-division multiplexing of phase-shifted holograms. Parallel phase-shifting digital holographic microscopy is implemented by an optical interferometer and an image sensor on which polarization-detection function is introduced pixel by pixel. This time, we constructed a parallel phase-shifting digital holographic microscope for recording high-speed dynamic phenomena, and then motions of biological objects in water were recorded at more than 10,000 frames per second, which is the fastest among the previous reports on 3-D imaging of biological objects.