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Saturable scattering and its application to superresolution microscopy

机译:饱和散射及其在超分辨显微镜中的应用

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Recently, several superresolution methods have been demonstrated to revolutionize the way we access the nano-world. However, these techniques are based on switching or saturation of fluorescence, and consequently are limited by switching reversibility and photobleaching. Here we show first superresolution far-field imaging based on scattering, which is a universal phenomenon without bleaching. Our principal finding is that scattering from plasmonics particles is saturable. By extracting the saturated part, sub-80-nm resolution is achieved. This work not only expands the horizon of superresolution imaging from fluorescence to scattering, but also points out the possibility of resolution enhancement for all imaging modalities that utilize plasmon properties.
机译:最近,已经证明了几种超分辨率方法,彻底改变了我们访问纳米世界的方式。但是,这些技术基于荧光的切换或饱和,因此受到切换可逆性和光漂白的限制。在这里,我们展示了基于散射的第一个超分辨率远场成像,这是一种没有漂白的普遍现象。我们的主要发现是,等离激元粒子的散射是可饱和的。通过提取饱和部分,可实现低于80 nm的分辨率。这项工作不仅将超分辨率成像的视野从荧光扩展到散射,而且还指出了提高所有利用等离激元特性的成像方式的分辨率的可能性。

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