Using Confocal Raman Microscopy to Investigate the Interaction Between Volatile Organic Compound (VOC) and Bovine Milk Fat Globules in Recombined Emulsion System

摘要

Confocal Raman microscopy (CRM) was applied to characterize the chemical profile of milk fat globules (MFGs) in recombined emulsion systems. Raman spectra of six different pure volatile organic compounds (VOCs) had been scanned under CRM. The interactions between VOCs (trans-2-hexenal) and individual MFGs in oil-water emulsion systems were successfully observed. Stronger intensities of characteristic Raman spectra of trans-2-hexenal were found on the MFGs than the milk fat globule membrane (MFGM) region in a recombined emulsion system after reaching system equilibrium, which reveals that the MFG as a whole, rather than MFGM, plays an important role in VOC absorption processes.