Ion Cyclotron Bioresonance in Regenerative Medicine

摘要

The prometheus myth, is a fitting model for regenerative medicine. As punishment for giving fire to humanity, Zeus ordered Prometheus chained to a rock and sent an eagle to eat his liver each day. However, Prometheus' liver was able to regenerate itself daily, enabling him to survive. Today we hope to make the legendary concept of regeneration into reality by developing therapies to restore lost, damaged, or aging cells and tissues in the human body. Electromagnetic therapy is a treatment method in which an electromagnetic or magnetic stimulus is used to achieve physiological changes in the body. The specific aim of the present work concerns the effectiveness of low frequency electromagnetic fields treatment (tuned at Calcium cyclotron energy resonance) to modify biochemical properties and trigger cells differentiation in a pituitary cells line (AtT20). Cells were exposed to a 7 Hz electromagnetic field (B_o field 9.2 μT) a commercially available wave generator (Vega Select 719), the cyclotron frequency were calculated by the following equation f_c = q/2πmB_0, where f_c is the cyclotron frequency, q and m are the charge and mass of the ion, and B_o is the vector of the geomagnetic field (DC field) parallel to the component of the applied electromagnetic field (B sin). In our case since the geomagnetic component (B_o) parallel to the applied Ssin is 9.2 μT, the calculated f_c for calcium is 7 Hz. Here, we report that 50 Hz 2mT ELF-EMF on rat anterior pituitary derived AtT20 D16V cells produces a sudden increase in the intracellular calcium level, followed by the reorganization of the cytoskeletal network via the polymerization of the actin and the differentiation of the proteins expression. These findings demonstrate that exposure to cyclotron resonance can transfer biological information on pituitary cells, supporting the relevance of low frequency electro-magnetic field as a therapeutic agent, thus suggesting the potential use of cyclotron resonance in nerve regeneratrion.